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You can develop mycelium on cardboard, although some fungi won’t develop fruiting bodies on it. You have to have a wood loving fungi, like oyster mushrooms, to successfully grow mushrooms from cardboard. On the other hand, it can be used as an agar substitute for almost every fungus. Let me tell you how.
You have a mushroom you want to clone. First of all you’ll want to cut the cap off to make a spore print. You now have only the stem left to work with.
Cut the stem in smaller pieces. You can leave them intact, but cutting them in pieces will make later transplantations to other substrates easier. Use a sterile knife for this, preferably a sterile razor blade or scalpel. Wear latex gloves or wash your hands with alcohol and put on a mouth mask.
Try not to bruise the mushroom too much, as my experience has taught me that bruises take longer too develop mycelium, or don’t develop mycelium at all. So be careful. Try to touch the mushroom stem as little as possible.
Now you have to prepare your cardboard. We do this by providing it with the correct moisture content. Put the cardboard in boiling water for about 1 or 2 minutes. Take it out and let it drip. Shake off as much of the access water as you can. When held vertically, the cardboard shouldn’t be dripping any more water.
Place the stems on your piece of cardboard and put everything in a ziplock bag.
Store the bag in a dark place at the right temperature. For most edible and hallucinogenic mushrooms 22°C should be fine. After a week you should get this as a result (see picture on the left). You can then cut little pieces of the cardboard using your sterile knife and distribute them among your substrates. Or you can use the other agar techniques to isolate a strong monostrain.
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I’m just now starting to get into all of this, and I’m a little confused…
I’m trying oysters for my first attempt and wondering why i keep reading about all these transitions from agar/cardboard to spawn and then to substrate? I’m trying to use cardboard, and wondering why I can’t just use cardboard as my “bulk” substrate and place my oyster stems right to that to begin with, let the mycelium populate and let them fruit in the same place? Maybe it’s a dumb question, but I’m not understanding the reason fr all the shuffling around. Thanks in advance.
You can grow on cardboard, but straw or wood is preferred. It has more mass and therefor yields more mushrooms.
Would this cardboard method work well for cloning a wild reishi fruitbody, seeing how it is a wood eating species?
Yes it would word, but cloning on agar petri dishes is always better.
Hi
I am new to Mushroom growing and i started with the White button using a spore print.
peat moss, spore prints, moist, and warm place.
now my question,
can i use the cloned cardboard to start the mushroom farm?
if yes,
shouldnt it be better that the mycelium (or at least easier to prepare?)
Thanks in advance.
You’re better of using the colonized cardboard to inoculate grain spawn. When the spawn is colonized, use that to inoculate your substrate. This cardboard method is just handy to cultivate or isolate mycelium.
Thank you very much
will give it a try one i get my first mushroom flush
i have a small question
i can see that there some kind of mycelium in the farm
and since this is my first time
i check on the net and find our that its color should be White
mine is Grey
is this normal, or does this mean that my farm is not going to grow
You probably have a contamination. Email me a picture and I’ll check to make sure:
info@mushroompalace.com
Thank you very much
yes it is a contamination
i even have flies coming out of the farm
so i think i need to start over again.
Whoa! Yes indeed.
Your cultures should be white at all times and they should smell like fresh mushrooms. Next time you’re cooking some fresh button or shiitake mushrooms, get a whiff, so you know how your culture should smell.
Not a problem. I’m sorry, I did find it difficult to speak in english.
Thank you for all your responses Sir.
Yours sincerely
Thank you still reply to my letter.
If I may ask:
A. What are the advantages of cloning system with LC compared to the production of fruit bodies of Oyster.
2. (Sorry if I’m not used to listening to another question) What if my mushrooms organ cultures with LC.
Thank you very much.
Yours sincerely.
I’m sorry to be so rude, but your questions just don’t make any sense.
1. LC is a way of cultivating mushroom mycelium. It’s a step in the production process of mushrooms. There’s nothing to compare.
2. I don’t understand question 2.
Kind regards,
Mushroom Palace
Thank you very much Mr. Sneakers.
Please forget the last question no 3. May I know which country you live and what time. As I type this in Indonesia at 00.00.
Really helped me and my knowledge grew after reading the whole article you sir.
If you do not mind, I will continue to discuss with you. once again thank you very much.
Yours sincerely.
note:
I will immediately practice the pictures above.
I’m from Belgium (GMT+2).